This book constitutes the second volume of the proceedings of the IUPESM World Congress on Medical Physics and Biomedical Engineering 2025, held in Adelaide, Australia, from September 29 to October 4, 2025. It presents recent research and developments in the fields of medical physics and biomedical engineering. The contributions address a broad range of topics including algorithms and methods in biomedical signal processing, smart healthcare devices and their applications, biomedical device design and safety, advances in data science for healthcare, and professional issues such as topics in education and women in biomedical and clinical engineering, alike. The volume serves as a valuable reference for medical physicists, biomedical engineers, and healthcare professionals, offering insights into current challenges and emerging trends, and fostering interdisciplinary collaboration for future advancements in healthcare technology.