This second edition details new and updated techniques used to study disulfide bonds. Chapters guide readers through how disulfide bonds are classified, techniques used to study functional disulfides, allosteric disulfide bonds, and examples of how labile disulfide bonds are employed for new diagnostics and therapeutics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Functional Disulphide Bonds: Methods and Protocols, Second Edition serves as a vital guide to this evolving area of study.